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- Take up 0.8ml of the phage / S.epidermidis mixture into the syringe. Label the remainder 'before filtration'.
- Pass the rest of the mixture through the filter into a sterile tube. Label this 'after filtration'.
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- Make a set of dilutions of 'before' and 'after' samples from 10-1 to 10-6 in phage buffer.
- Assay for bacteria:
Spread 100µl of undiluted 'before' and 'after' samples onto nutrient agar plates.
- Assay for phage:
Carry out a phage count on the 'before' and 'after' samples, as described elsewhere.
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- When the plates are dry, incubate overnight at 37oC.
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