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- Add 0.1ml E.coli suspension (plating cells) to each of the dilution tubes. (Why?)
- Remove one tube of soft agar from the 45°C water bath.
- Add 0.1ml of the 10-6 phage dilution to the agar.
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- Mix thoroughly but gently (no bubbles!) and pour onto one of the agar plates.
- Swirl gently to distribute the agar evenly across the plate.
- Repeat this operation for each of the phage dilutions in this series.
- Repeat the entire procedure above to test for total p.f.u. from the chloroform-lysed sample.
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Put your plates into the incubator overnight at 37°C
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