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4) Place the plate in the plate washer and wash the wells three times with PBS. |
| 5) Add 200µl of the enzyme-conjugated anti-IgG and anti-IgM sera
to the appropriate wells. Incubate
the plate for a further 1 hour at 37°C in the humidified box.
6) Wash the plate as before (step 4). Add 200µl of the enzyme substrate (p-nitrophenyl phosphate) to all the wells. Incubate the plate at 37°C for a further 20 min. 7) Stop the reaction by the addition of 50µl 3M NaOH to all the wells. |
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