Here are your samples:

Carefully remove 10µl of each reaction (the aqueous layer beneath the mineral oil) and place in a fresh Eppendorf tube.
DO NOT TAKE UP ANY MINERAL OIL (or your sample will "float" when you try to load the gel).
  • To each tube, add 10µl gel loading buffer.

  • Load the samples on a 2% agarose gel.

  • Also load the 100bp "size marker" sample provided.

  • Remember which lane is which!

Run the gel


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