Influenza Haemagglutination: Page 5/23

Haemagglutination Inhibition Assay:

	Put 50µl of PBS into the following wells of the microtitre plate: C1-C10 D1-D10 E1-E10 Put 50µl of antiserum in the first well of each row (use one row for each antiserum). Mix and transfer 50µl to second well, and so on, making doubling dilutions all the way down the row. Discard 50µl from the last well in each row. Positive control for haemagglutination: well F1 = 50µl 'Flu' + 50µl RBC's. Add 50µl influenza virus preparation (= 8 HAU) to all the wells - work from right to left - why? Add 50µl RBC's to all the wells - work from right to left - why? Mix the cell suspensions by tapping the plate gently.
Click here to see how to set up this assay

Put 50µl of PBS into the following wells of the microtitre plate:
C1-C10
D1-D10
E1-E10
Put 50µl of antiserum in the first well of each row (use one row for each antiserum). Mix and transfer 50µl to second well, and so on, making doubling dilutions all the way down the row. Discard 50µl from the last well in each row.
Positive control for haemagglutination: well F1 = 50µl 'Flu' + 50µl RBC's.
Add 50µl influenza virus preparation (= 8 HAU) to all the wells - work from right to left - why?
Add 50µl RBC's to all the wells - work from right to left - why?
Mix the cell suspensions by tapping the plate gently.