Since the sampling intervals are critical, we will perform this as a group experiment.
I will take samples from the bacteriophage-infected culture at 3 minute intervals.
Half of each sample will be lysed with chloroform to assay the total p.f.u. (i.e. free phage plus any infectious intracellular phage particles). The other half will be used to assay the free (extracellular) p.f.u.
(N.B. This bacteriophage is resistant to chloroform, but the bacterial cells are not!)
You will analyse the free/total p.f.u. from a single time point - 60 minutes. At the end of the experiment we'll pool all the results so that you can plot graphs.
You are provided with the following:
