The A280 for BSA is 1.0 at 1.9mg/ml.
Calculate the A280 for antigen at equivalence:
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A) Amount of antigen = 20µl = 0.2mg Concentration in cuvette = 0.1mg/ml If 1.9mg/ml BSA gives an A280 of 1.0, then A280(Ag) = (0.1 x 1)/1.9 = 0.19. |
B) Amount of antigen = 0.02 x 4 (mg/ml BSA) = 0.08mg Concentration in cuvette = 0.08mg/ml If 1.9mg/ml BSA gives an A280 of 1.0, then A280(Ag) = (0.08 x 1)/1.9 = 0.042. |
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C) Amount of antigen = 0.02 x 4 (mg/ml BSA) = 0.08mg Concentration in cuvette = 0.04mg/ml If 1.9mg/ml BSA gives an A280 of 1.0, then A280(Ag) = (0.04 x 1)/1.9 = 0.021. |
D) Amount of antigen = 0.02 x 4 (mg/ml BSA) = 0.08mg Concentration in cuvette = 0.04mg/ml If 1.9mg/ml BSA gives an A280 of 1.0, then A280(Ag) = 0.04 x 1.9 = 0.076. |