0.244 x 0.7 (mg IgG=A280 1.0) = 0.17mg/ml.
0.17 x 2 (dilution in cuvette) = 0.34mg IgG.
100µl of antiserum were used per tube, therefore the concentration of the antibody in the original antiserum is 0.34 x 10 = 3.4mg/ml.
Estimate the number of antigenic determinants on a molecule of BSA