LabWork

Antibody-Antigen Interactions

One of the first observations of antigen-antibody interactions was their ability to precipitate when combined in certain proportions. The quantitative precipitin test relies on this fact. This test forms the basis of all quantitative studies of antigen-antibody interactions. Increasing amounts of antigen are added to a constant amount of antibody and the weight of precipitate formed in each tube is determined.

In this experiment, you will determine:

1. The antibody concentration of a test antiserum
2. The number of antigenic determinants (antibody binding sites) on an antigen molecule.

• Rabbit anti-BSA antiserum of unknown titre (concentration) - the antibodies
• Bovine serum albumin, (4mg/ml) - the antigen
• Phosphate buffered saline (PBS) pH 7.2
• 0.1M NaOH

Perform the experiment  

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